2-hydroxyisobutyryl-HIST1H1C (K128) Antibody

Immunocytochemistry analysis of CSB-PA010378OA128hibHU diluted at 1:50 and staining in Hela cells (treated with 30mM sodium butyrate for 4h) performed on a Leica BondTM system. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4?C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.